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研究丁香苷对佐剂性关节炎(AA)大鼠的治疗作用及其机制。采用大鼠足跖皮内注射弗氏完全佐剂诱发大鼠AA模型。足容积法测量继发侧足肿胀度,进行疼痛评分和多发性关节炎评分,MTT法检测刀豆蛋白(ConA)和脂多糖(LPS)诱导的脾淋巴细胞增殖反应,酶联免疫吸附测定法(ELISA)测定ConA诱导的脾淋巴细胞产生IL-2的水平以及脂多糖(LPS)诱导的腹腔巨噬细胞(PMΦ)产生IL-1β和TNF-α的水平。结果表明,大鼠致炎后第14天,继发性关节炎出现,同时灌胃给予不同剂量的丁香苷及阳性对照药雷公藤多苷,连续给药16天。丁香苷各剂量组于第22天开始不同程度降低AA大鼠继发侧关节肿胀度、关节疼痛评分、多发性关节炎指数;同时各剂量组可不同程度地提高AA大鼠低下的脾淋巴细胞增殖反应和脾细胞IL-2的产生,降低AA大鼠PMΦ产生过高的IL-1β和TNF-α。这些结果提示,丁香苷可以改善AA大鼠异常的细胞免疫功能并调节细胞因子的平衡,可能是其治疗AA大鼠继发性炎症的作用机制。
To study the therapeutic effect of syringin on adjuvant arthritis (AA) rats and its mechanism. Rat AA model was induced by intradermal injection of Freund’s complete adjuvant in rat foot. Foot volume method was used to measure the degree of secondary collateral swelling. The pain scores and multiple arthritis scores were measured. The proliferation of splenic lymphocytes induced by ConA and LPS was detected by MTT assay. The enzyme linked immunosorbent assay The levels of IL-2 produced by ConA-induced spleen lymphocytes and the levels of IL-1β and TNF-α produced by lipopolysaccharide (LPS) -induced peritoneal macrophages (PMΦ) were determined by ELISA. The results showed that on the 14th day after inflammation in rats, secondary arthritis appeared. At the same time, different dosages of syringin and the positive reference drug Tripterygium glycosides were given intragastrically for consecutive 16 days. In each dose group of syringin, the degree of secondary joint swelling, joint pain score and multiple arthritis index in AA rats were decreased by different degrees on the 22nd day. At the same time, each dose group could improve the splenic lymphocytes Proliferative response and the production of spleen cells IL-2, reduce excessive production of IL-1β and TNF-α in PMΦ of AA rats. These results suggest that syringin can improve the abnormal cellular immune function and regulate the balance of cytokines in AA rats, which may be the mechanism of its effect on secondary inflammation in AA rats.