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目的:研究CK-MB在反映新生儿窒息所致的脑损伤中的临床意义及诊断价值。方法:收集49例实验组及20例对照组,其中将实验组分成合并脑损伤(27例)和不合并脑损伤(22例)两对比组,分别采用自动生化分析仪法与琼脂糖凝胶电泳法检测其总CK活性、同工酶活性和CK-MB%。结果:(1)对照组两方法测定结果比较,自动生化分析仪法测定的CK-MB%高于琼脂糖凝胶电泳法(简称电泳法)检测的结果(P<0.01)。实验组中自动生化分析仪测定的CK-MB%高于电泳法检测的结果(P<0.01)。对照组和实验组比较,CK-MB%测定结果无差别(P>0.05)。(2)实验组中合并脑损伤组中CK-MB%和不合并脑损伤组比较,测定结果无差别(P>0.05)。结论:自动生化分析仪法测定的CK-MB%明显高于电泳法检测的CK-MB%。上述两种方法检测的CK-MB%在实验组和对照组间以及在新生儿窒息中是否合并脑损伤并无显著性差异,不宜作为诊断新生儿窒息所致的脑损伤的生化指标。
Objective: To investigate the clinical significance and diagnostic value of CK-MB in reflecting brain injury induced by neonatal asphyxia. Methods: 49 cases of experimental group and 20 cases of control group were collected. The experimental group was divided into two groups: control group with brain injury (n = 27) and non-brain injury group (n = 22). The rats were divided into two groups by automated biochemical analyzer and agarose gel The total CK activity, isozyme activity and CK-MB% were detected by electrophoresis. Results: (1) The CK-MB% of the two methods in the control group was higher than that of the agarose gel electrophoresis (abbreviated as electrophoresis) (P <0.01). The CK-MB% of automatic biochemical analyzer in the experimental group was higher than that of electrophoresis (P <0.01). There was no difference between the control group and experimental group (P> 0.05). (2) There was no difference between CK-MB% and brain injury group in the experimental group with brain injury group (P> 0.05). Conclusion: The CK-MB% measured by automated biochemical analyzer was significantly higher than CK-MB% detected by electrophoresis. The CK-MB% detected by the above two methods had no significant difference between the experimental group and the control group and with or without neonatal asphyxia, and should not be used as a biochemical index to diagnose brain injury caused by neonatal asphyxia.