目的:建立阿魏酸哌嗪有关物质的测定方法。方法:采用HPLC法,色谱柱:Xtimate C_(18)柱(250 mm×4.6 mm,5μm);流动相:0.5%冰乙酸溶液-甲醇-乙腈,采用梯度洗脱;流速:1.0 ml·min~(-1);柱温:30℃;检测波长:286 nm;进样量:20μl。结果:阿魏酸在5~30μg·ml~(-1)(r=1.000 0)浓度范围内线性关系良好,检测限为0.02 ng。结论:本测定方法可靠、简便、结果准确,稳定性和耐用性良好,适用于阿魏酸哌嗪有关物质的测定。 Objective: To establish a method for the determination of piperazine ferulate. Methods: The HPLC method was adopted. The column was Xtimate C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.5% glacial acetic acid and methanol - acetonitrile with a gradient elution. The flow rate was 1.0 ml · min ~ (-1); column temperature: 30 ℃; detection wavelength: 286 nm; injection volume: 20μl. Results: Ferulic acid had a good linearity in the concentration range of 5 ~ 30μg · ml -1 (r = 1.000 0) with a detection limit of 0.02 ng. Conclusion: The method is reliable, simple, accurate, stable and durable. It is suitable for the determination of piperazine ferulate.