建立陈皮中总黄酮的含量测定方法,以控制其质量。以橙皮苷为对照品,采用ZrOCl2比色法测定陈皮中总黄酮的含量,测定波长为312.5nm。在浓度0.0044—0.022mg/mL范围内,吸光度与浓度呈良好的线性关系(r=0.9999),平均回收率为100.69%,RSD为1.64%(n=6)。本法操作简单,具有良好的稳定性和重现性,适用于陈皮中总黄酮的含量测定和评价。 To establish the method of determination of total flavonoids in dried tangerine peel to control its quality. Hesperidin was used as reference substance, and the content of total flavonoids in dried tangerine peel was determined by ZrOCl2 colorimetry. The wavelength was 312.5 nm. In the range of 0.0044-0.022mg / mL, there was a good linear relationship between absorbance and concentration (r = 0.9999), the average recovery was 100.69% and the RSD was 1.64% (n = 6). The method is simple to operate, has good stability and reproducibility, and is suitable for the determination and evaluation of the content of total flavonoids in dried tangerine peel.