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目的探讨结核蛋白芯片用于肺外结核辅助诊断的临床应用。方法应用蛋白芯片对146例肺外结核患者及130例健康对照组标本,进行结核菌蛋白16KDa和38KDa以及脂阿拉伯甘露糖(LAM)三种结核特异性抗原得相应抗体检测,同时与ELISA法检测的血清TBAb进行对照。结果结核蛋白芯片阳性率为76.7%,较ELISA法(60.9%)有显著差异(χ2=21.04,P<0.01),三种抗体阳性率以抗LAM最高,占64.3%,依次是抗38KDa抗体53.4%、抗16KDa抗体27.3%。结论结核蛋白芯片用于肺外结核辅助诊断具有较高的灵敏性和特异性,具有一定的临床应用价值。
Objective To investigate the clinical application of tuberculosis protein chip in the auxiliary diagnosis of extrapulmonary tuberculosis. Methods 146 cases of extrapulmonary TB patients and 130 healthy controls were enrolled in this study. The corresponding antigens were detected by TB Mycobacterium tuberculosis proteins 16KDa, 38KDa and LAM. At the same time, Serum TBAb was compared. Results The positive rate of tuberculosis protein chip was 76.7%, which was significantly different from that of ELISA method (60.9%) (χ2 = 21.04, P <0.01). The highest positive rate of anti-LAM was 64.3%, followed by anti-38KDa antibody 53.4 %, Anti-16KDa antibody 27.3%. Conclusion Tuberculosis protein chip for pulmonary tuberculosis auxiliary diagnosis with high sensitivity and specificity, has a certain clinical value.