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本研究利用CRISPR/Cas9技术对水稻OsGPRP家族基因进行敲除,在3个水稻OsGPRP基因的第一个外显子PAM序列区域各设计一个靶位点,并将3个靶位点的sg RNA进行串连,成功构建了3个OsGPRP基因同时敲除的CRISPR/Cas9载体;采用根癌农杆菌介导的遗传转化获得了60株T0代CRISPR转基因植株;通过PCR扩增和测序分析,我们获得了2种不同类型的OsGPRP3单基因突变材料;6种不同类型的OsGPRP2/OsGPRP3两基因突变植株;1种类型的OsGPRP1/OsGPRP3两基因纯合突变植株和4种不同类型的OsGPRP1/OsGPRP2/OsGPRP3三基因突变材料,为进一步研究OsGPRP家族基因的生物学功能及其相互关系提供遗传材料.“,”In this study, OsGPRPs family genes in rice were knocked out based on CRISPR/Cas9 technology. We designed a target site in PAM sequence region in the first exon of 3 OsGPRPs genes in rice correspondingly and serialized the sg RNA on 3 target sites. Later we successfully constructed CRISPR/Cas9 vectors that knocked out with 3 OsGPRPs genes. 60 T0 CRISPR transgenic plants were obtained through Agrobacterium-mediated genetic transformation. Target PCR amplification and sequencing were applied to these plants. We obtained two single gene mutant plants of OsGPRP3 in different types, 6 mut plants of OsGPRP2/OsGPRP3 genes in different types, homozygous mutant plant of OsGPRP1/OsGPRP3 genes in one type and 4 mutant plants of OsGPRP1/OsGPRP2/OsGPRP3 genes in different types. This work would provide the genetic material for further researches on the biological function and interrelationships of OsGPRPs family genes.