应用游离小孢子培养技术,培养茄子体细胞融合杂种DSa不同株系的小孢子。结果表明:对花药进行3C℃高温6 d预处理,能显著提高小孢子脱分化能力;小孢子离体培养的最佳发育时期是单核期;小孢子培养的适宜培养基为KM附加PEG 250 mg/L、2,4-D 0.2 mg/L、ZT 0.5 mg/L、NAA 1mg/L及6.5％的葡萄糖;再生植株适宜培养基为MS附加ZT 2 mg/L、IAA 0.1 mg/L、2％蔗糖和7g/L琼脂。 The microspores of different strains of hybrid DSa were cultivated with free microspore culture. The results showed that pretreatment of anther at 6 ℃ for 6 d could significantly improve the ability of microspore dedifferentiation. The optimal microspore culture period was monocyte stage. The suitable medium for microspore culture was KM plus PEG 250 L, 2,4-D 0.2 mg / L, ZT 0.5 mg / L, NAA 1 mg / L and 6.5% glucose. The appropriate medium for regeneration was MS supplemented with ZT 2 mg / L and IAA 0.1 mg / 2% sucrose and 7 g / L agar.