恶性疟P190(亦称裂殖子表而抗原1,MSA1)是疟原虫红内期裂殖体产生的一种塘蛋白,分子量约190 kD。由于该抗原在疟疚病人和实验动物中均能诱生很强的保护性免疫力,因而已将它列入重要的疟疾疫苗候选抗原。P190存在株间抗原差异,但该变异属于等位基因双态变异。至今经序列分析鉴定的虫株中只发现两种变异类型序列,MAD20型和Kl型。比较不同虫株该基因序列可将该分子划分为保守区、双态区和多态区。近来研究提示,双态区可能是该分子重要保护性免疫控制区。我们克隆了我国恶性疟FCC1/HN株该双态区的基因。方法为设计和合成一对引物,在引物的5’端设有 Plasmodium falciparum P190 (also known as merozoite antigen 1, MSA1) is a pond protein produced by the red blood cells of the malaria parasite and has a molecular weight of about 190 kD. Since this antigen induces a strong protective immunity in both malaria patients and laboratory animals, it has been included as an important malaria vaccine candidate antigen. P190 there are differences between the strains of antigens, but the mutation belongs to allelic binary state variation. Up to now, only two mutation types, MAD20 and Kl, have been found in insect strains identified by sequence analysis. Comparison of different insect strains of the gene sequence can be divided into the conserved region of the molecule, dual-state and polymorphic regions. Recent studies suggest that the bimodal region may be the important protective immune control region of the molecule. We cloned the gene of the binary region of P. falciparum FCC1 / HN strain in China. The method is to design and synthesize a pair of primers, which are provided at the 5 ’end of the primer