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为探讨即早反应基因（ｉｍｍｅｄｉａｔｅｅａｒｌｙｇｅｎｅｓ，ＩＥＧｓ）在神经损伤过程中的变化规律，用原位分子杂交和ＰＡＰ免疫组织化学方法观察了大鼠脊髓内注射红藻氨酸（ｋａｉｎｉｃａｃｉｄ，ＫＡ）后１ｈ至１４ｄ的不同时间点，Ｌ１～３脊髓腹、背角神经元中ｃ－ｆｏｓｍＲＮＡ和Ｆｏｓ免疫阳性信号的变化。结果表明，ＫＡ致脊髓损伤后２～８ｈ脊髓腹角运动神经元中ｃ－ｆｏｓｍＲＮＡ的表达和Ｆｏｓ免疫组织化学阳性反应明显增强，１２ｈ恢复至正常水平，伤后３ｄ又明显增强；而脊髓背角神经元内仅在伤后２～６ｈ明显增加。提示ＫＡ对脊髓神经元的选择性毒性作用；运动神经元ｃ－ｆｏｓ表达的再次增强与ＫＡ所致神经元的不可逆变性有关，亦可看作是神经元损伤后死亡的先兆之一。
To investigate the changes of immediateearlygenes (IEGs) during nerve injury, in situ hybridization and PAP immunohistochemistry were used to observe the changes of kainic acid (KA) 14d at different time points, L1 ~ 3 spinal cord dorsal horn neurons c-fos mRNA and Fos immunoreactive signal changes. The results showed that the expression of c-fos mRNA and the positive Fos immunohistochemical reaction in KV neurons of spinal cord ventral horn increased from 2 to 8 h after spinal cord injury induced by KA, returned to the normal level at 12 h, and significantly increased on the 3rd day after spinal cord injury. However, Neurons within 2 ~ 6h after injury increased significantly. Suggesting the selective toxicity of KA to spinal neurons. The re-enhancement of c-fos expression in motor neurons is related to the irreversible degeneration of neurons induced by KA, which may also be regarded as one of the precursors of neuronal death after injury.