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本研究用离体培养的人淋巴细胞为材料,观察外源性SOD和减少细胞内源性SOD对电离辐射诱发染色畸变的效应。实验结果表明,辐射诱发的染色体畸变除了射线的直接“击中”效应外,也与自由基的间接效应有关。因此,向受照射的淋巴细胞的培养物中加入外源性的超氧化物歧化酶(SOD)以清除损伤DNA的超氧自由基(O_2~-),可减少辐射诱发的染色体损伤。反之向受照射的淋巴细胞的培养物中引入SOD的抑制剂(DDC),则可增加淋巴细胞的辐射敏感性,加剧辐射对染色体的损伤作用。
In this study, human lymphocytes cultured in vitro were used as materials to observe the effects of exogenous SOD and reducing endogenous cellular SOD on ionizing radiation-induced staining. The experimental results show that the radiation-induced chromosome aberrations are related to the indirect effects of free radicals in addition to the direct “hitting” effect of the rays. Therefore, adding exogenous superoxide dismutase (SOD) to the irradiated lymphocyte culture to eliminate DNA damage superoxide radical (O 2 -) can reduce radiation-induced chromosome damage. Conversely, the introduction of SOD inhibitors (DDC) into irradiated lymphocyte cultures increases the radiation sensitivity of lymphocytes and exacerbates the damaging effects of radiation on chromosomes.