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目的探讨片仔癀联合多西他赛对舌鳞状细胞癌(鳞癌)Tca8113细胞生长、凋亡及细胞周期的影响。方法取对数生长期Tca8113细胞,设对照组、片仔癀单药组(450μg/m L)、多西他赛单药组(0.01μg/L)及联合用药组,培养48 h后,应用碘化丙啶(propidium iodide,PI)染色检测药物对Tca8113细胞周期的影响,应用流式细胞仪检测细胞的凋亡情况。结果 PI染色结果显示片仔癀与多西他赛联合用药可阻滞细胞于G0/G1期和G2/M期,且与对照组及各单药组相比S期细胞的下降比例显著降低(P<0.01)。Annexin V/PI双染试验结果显示联合用药组与对照组及各单药组相比,细胞凋亡比例显著升高(P<0.01)。结论片仔癀与多西他赛联合应用可抑制舌鳞癌细胞生长并促进细胞凋亡。
Objective To investigate the effect of Pien Tze Huang combined with docetaxel on the growth, apoptosis and cell cycle of tongue squamous cell carcinoma (SCC) Tca8113 cells. Methods The Tca8113 cells in logarithmic growth phase were treated with iodized solution (control group, Pien Tze Huang single drug group (450μg / m L), docetaxel monotherapy group (0.01μg / L) Propidium iodide (PI) staining was used to detect the effect of drugs on the cell cycle of Tca8113 cells. The apoptosis of cells was detected by flow cytometry. Results The results of PI staining showed that the combination of Pien Tze Huang and docetaxel blocked the cells in G0 / G1 phase and G2 / M phase, and the proportion of S phase cells decreased significantly compared with control group and single drug group (P < 0.01). The results of Annexin V / PI double staining showed that the apoptosis ratio in the combination group was significantly higher than that in the control group and the single drug group (P <0.01). Conclusion Pien Tze Huang combined with docetaxel can inhibit tongue squamous cell carcinoma cell growth and promote apoptosis.