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目的以致敏绵羊红细胞为工具研究肠道病毒71型(EV71)的细胞嗜性,并探讨传代培养细胞表面EV71受体表达情况。方法 EV71致敏的绵羊红细胞与传代培养细胞作用,观察不同方法处理的培养细胞吸附致敏绵羊红细胞的情况,分析EV71的细胞嗜性,并探讨培养细胞表面EV71受体的表达情况。结果 EV71致敏绵羊红细胞能吸附在人喉癌上皮细胞(Hep-2)、恒河猴胚肾细胞(MA104)的细胞表面,并且胰酶能破坏MA104的吸附功能,对Hep-2却无影响,而狗肾细胞(MDCK)与EV71致敏的绵羊红细胞未见吸附现象,这与病毒直接感染细胞结果一致。结论该方法能直接、有效地反映病毒的细胞嗜性及培养细胞表面病毒受体的表达情况,在病毒学尤其是病毒受体研究中具有较强的可行性及应用价值。
Objective To study the cell tropism of enterovirus 71 (EV71) by using sensitized sheep erythrocytes and to investigate the expression of EV71 receptor on the surface of subcultured cells. Methods EV71 sensitized sheep erythrocytes were cultured with subcultured cells. The sensitized sheep erythrocytes were cultured with different methods. The EV71 cell tropism was analyzed, and the expression of EV71 receptor on cultured cells was also investigated. Results EV71 sensitized sheep red blood cells could adsorb on the cell surface of human laryngeal epithelial cells (Hep-2) and rhesus embryo kidney cells (MA104), and trypsin could destroy the adsorption function of MA104, but had no effect on Hep-2 , While there was no adsorption phenomenon between dog kidney cells (MDCK) and EV71 sensitized sheep erythrocytes, which was consistent with the direct virus infection of the cells. Conclusion The method can directly and effectively reflect the cell tropism of virus and the expression of virus receptor on cultured cell surface, and has strong feasibility and application value in the study of virology, especially virus receptor.