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目的建立归芪消白胶囊中二苯乙烯苷和丹皮酚的含量测定方法。方法采用Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相,梯度洗脱,检测波长:320 nm,流速:1.0 ml·min~(-1),柱温:35℃,进样量:10μl。结果二苯乙烯苷和丹皮酚在5.0~200.0μg·ml~(-1)范围内与峰面积均呈良好线性关系,二苯乙烯苷和丹皮酚的平均回收率分别为97.1%、96.2%,RSD均为3.4%。结论本方法简便、快捷,可作为归芪消白胶囊的质量控制方法。
Objective To establish a method for the determination of stilbene glycoside and paeonol in Guiqi Xiaobai Capsule. Methods Kromasil C 18 column (250 mm × 4.6 mm, 5 μm) was used with gradient elution with acetonitrile-water as the mobile phase. The detection wavelength was 320 nm, the flow rate was 1.0 ml · min -1, Temperature: 35 ℃, injection volume: 10μl. Results The calibration curves of stilbene glycoside and paeonol showed a good linear relationship with the peak area in the range of 5.0 ~ 200.0μg · ml ~ (-1). The average recoveries of stilbene glycoside and paeonol were 97.1%, 96.2 %, RSD are 3.4%. Conclusion The method is simple and rapid, and can be used as quality control method of Guiqi Xiao Bai Capsule.