材料瘤株:小鼠肉瘤S_(180)。动物用昆明种小白鼠。光源1.GGH 400型黑光照尉器,波长3200(?)～4000(?),主峰在3650(?),功率400w,长沙医疗公司机械厂制造;2.氦镉激光器,波长4416(?),功率70～100mw,西北大学研制。药物:1.补骨脂素,三军大新桥医院药局从中药补骨脂中分离、提纯、鉴定,批号860506;2.8—甲氧基补骨脂素(8—MOP),湖南医药工业研究所标准品。方法1.补骨脂素加光对肉瘤细胞的杀伤:取接种6天后小鼠S_(180)腹水型细胞,生理盐水洗涤2次,用pH7.4的1640液稀释为每毫升含数2×10~(7)瘤细胞悬液,每组取3.75ml 置30mm 平器中,共分为6组,分组处理如下:1.1对照组:瘤细胞悬液3.75ml+药物溶剂1.25ml,37℃温育90min。1.2光照组:瘤细胞悬液3.75ml+药物溶剂1.25m1,37℃温育60min,照光30min。1.3 8—甲氧基补骨脂素组:瘤细胞悬液 Material tumor strain: mouse sarcoma S_(180). Kunming mice were used for animals. Light source 1.GGH 400 type black light irradiation device, wavelength 3200(?)-4000(?), main peak at 3650(?), power 400w, manufactured by Changsha Medical Corporation Machinery Factory; 2. helium cadmium laser, wavelength 4416(?) , Power 70 ~ 100mw, developed by Northwest University. Drugs: 1. Psoralen, separation, purification, identification of traditional Chinese medicine psoralen from Daxinqiao Hospital of the Third Armies, batch No. 860506; 2.8—methoxypsoralen (8-MOP), Hunan Pharmaceutical Industry Research The standard product. Method 1. Psoralen plus light to kill sarcoma cells: Six days after inoculation, mouse S180 ascites cells were washed twice with physiological saline and diluted with 1640 solution at pH 7.4 to 2× per milliliter. 10 ~ (7) tumor cell suspension, each group to take 3.75ml set 30mm flatware, are divided into 6 groups, grouped as follows: 1.1 control group: tumor cell suspension 3.75ml + drug solution 1.25ml, 37 °C incubation 90min. 1.2 Illumination group: tumor cell suspension 3.75 ml + drug solvent 1.25m1, incubated at 37°C for 60 minutes, illuminated for 30 minutes. 1.3 8-methoxypsoralen group: tumor cell suspension