论文部分内容阅读
研究建立了一套简单、高效的辣椒遗传转化系统。利用根癌农杆菌介导的带柄子叶转化法将豇豆胰蛋白酶抑制剂 (CpTI)基因转入辣椒栽培品种益都羊角椒中。对获得的 3 3株卡那霉素 (Km)抗性再生植株进行PCR检测 ,证明有 6株为转CpTI基因植株
The study established a simple and efficient system of pepper genetic transformation. The gene of cowpea trypsin inhibitor (CpTI) was transferred into Capsicum annuum L. by using Agrobacterium tumefaciens-mediated cotyledon transformation. Three 3 strains of kanamycin (Km) resistant regenerated plants were tested by PCR, and 6 plants were confirmed as CpTI transgenic plants