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近年来邻苯二甲酸酯污染已成为中国一个重要的环境问题.为探讨邻苯二甲酸二乙基己酯(di-(2-ethylhexyl)phthalate,DEHP)在诱发哮喘方面的作用,及研制DEHP诱导大鼠哮喘模型,将32只Wistar大鼠随机分成4组(每组8只):生理盐水对照组、卵清白蛋白(OVA)致敏组和2个DEHP染毒组,采用OVA致敏加激发的方法制作大鼠哮喘模型.2个DEHP染毒组大鼠每天分别进行0.7mg·kg-1和70mg·kg-1邻苯二甲酸二乙基己酯灌胃染毒,连续30d.OVA致敏组、DEHP染毒组大鼠均在第31～37天给予1%OVA雾化,诱发哮喘.第38天取肺脏做组织切片,进行分析.结果表明,与OVA组相比,DEHP染毒组气管壁增厚,细胞浸润增加,气道重塑,特别是在70mg·kg-1·d-1组,变化更为明显.由此可以得出结论,邻苯二甲酸二乙基己酯可诱导哮喘模型大鼠气道重塑,在诱发哮喘发病过程中可能起到佐剂作用。
In recent years, phthalate pollution has become an important environmental issue in China.In order to explore the role of di- (2-ethylhexyl) phthalate (DEHP) in the induction of asthma, DEHP induced rat asthma model, 32 Wistar rats were randomly divided into 4 groups (8 rats in each group): normal saline control group, OVA sensitized group and 2 DEHP treated groups, sensitized with OVA The rat model of asthma was made by adding stimulation method.The rats in two DEHP groups were orally administered with diethylhexyl phthalate (0.7 mg · kg-1 and 70 mg · kg-1) daily for 30 days. OVA sensitized group and DEHP exposed group were given 1% OVA atomization on the 31st to 37th days to induce asthma.On the 38th day, the lungs were taken for tissue section and analyzed.The results showed that compared with OVA group, DEHP The changes of tracheal wall thickening, cell infiltration and airway remodeling, especially in the group of 70 mg · kg-1 · d-1, were more obvious in the exposure group.Therefore, it can be concluded that diethyl phthalate Hexyl ester can induce airway remodeling in asthmatic rats and may play an adjuvant role in the pathogenesis of asthma.