研究绿藻中多糖硫酸酯的提取和纯化及其对小鼠的抗肿瘤活性。先用溶剂法提取,再用DEAE纤维素柱色谱和凝胶柱色谱分离纯化,制得纯品多糖硫酸酯。通过对移植性动物瘤株的抑瘤试验来验证其抗肿瘤活性。结果表明,(2～8)g/kg·d剂量对小鼠S-180的抑瘤率为42.3%～82.0%,p<0.001。对小鼠肝癌的抑瘤率为52.2%～86.1%,p值<(0.01～0.001)。 To study the extraction and purification of polysaccharide sulfate from Chlorella and its antitumor activity in mice. Firstly, it was extracted with solvent and then purified by DEAE cellulose column chromatography and gel column chromatography to obtain pure polysaccharide sulfate. The anti-tumor activity of the transplanted animal tumor strain was verified by the tumor inhibition test. The results showed that (2 ~ 8) g / kg · d dose of mice S-180 tumor inhibition rate was 42.3% ~ 82.0%, p <0.001. The tumor inhibition rate of mouse liver cancer was 52.2% to 86.1%, and the p value was <0.01-0.001.