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报道了杂色鲍碱性磷酸酶(ALP)在甲醇、乙醇、丙醇、甲醛、丙酮等溶液中的失 活动力学．结果表明:酶的剩余活力随着有机溶剂浓度的增大而迅速下降．测得上述 5种有机溶剂对该酶的半抑制率(IC50)分别为5．41、3．07、1．80、31．0×10-3、2．30 mol／dm3．在这些有机溶剂溶液中酶的失活过程都是可逆反应．检测醇、醛、酮对该酶 的失活作用机理,结果表明甲醇、乙醇、丙醇对杂色鲍ALP的失活作用均为非竞争性 机制,其抑制常数分别为5．36、3．02、1．80mol／dm3．甲醛、丙酮对杂色鲍ALP的失活 作用都呈现为混合型抑制,其对游离酶的抑制常数(K1)分别为24．7×10-3、1．66 mol／dm3;对酶-底物络合物的抑制常数(KIS)分别为56．2×10-3、5．40mol／dm3．
The inactivation kinetics of variegated alkaline phosphatase (ALP) in methanol, ethanol, propanol, formaldehyde and acetone solutions was reported. The results showed that the residual activity of the enzyme decreased rapidly with the increase of the organic solvent concentration. The half-inhibition rates (IC50) of these five organic solvents were 5.41, 3.07, 1.80, 31.0 × 10-3 and 2.30 mol / dm3, respectively. The enzymatic inactivation in these organic solvent solutions is reversible. The mechanism of inactivation of alcohols, aldehydes and ketones was investigated. The results showed that the inactivation of ALP by methanol, ethanol and propanol was noncompetitive and the inhibition constants were 5.36,3 and 5.36, respectively. 02, 1.80 mol / dm3. Formaldehyde and acetone inhibited the ALP inactivation in mixed Haliotis discus hannai, and the inhibitory constant (K1) of free enzyme was 24.7 × 10-3 and 1.66 mol / dm3 respectively. The complex inhibition constants (KIS) were 56.2 × 10-3, 5.40 mol / dm3, respectively.