IL-2是一个由133个氨基酸组成的多肽激素,在遇到抗原后T 细胞的增殖和扩大中需要这种激素。目前使用的测定IL-2受体的方法只能同时测定数量有限的标本。而且还必须进行在水浴中机械旋转,反复离心等繁复的步骤。作者研制了一种新的方法,这种方法使用经过预处理带有可移动孔的微量滴定板操作,可以用最少的试剂同时测定一些细胞系的结合参数。并且还可用于除IL-2以外的配位 IL-2 is a polypeptide hormone consisting of 133 amino acids that is required for the proliferation and expansion of T-cells following antigen challenge. The currently used method of determining IL-2 receptor only allows for the simultaneous determination of a limited number of specimens. But also must be carried out in the water bath mechanical rotation, repeated centrifugation and other complicated steps. The authors developed a new method that uses pretreated microtitre plates with movable wells to allow the simultaneous determination of binding parameters of some cell lines with a minimum of reagents. And can also be used for coordination other than IL-2