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建立了高效液相色谱法测定大鼠血浆中的甘草次酸,并考察其在大鼠体内的药动学。采用Symmetry~Shield C18色谱柱,以乙腈︰0.1%磷酸为流动相,梯度洗脱,检测波长254 nm。甘草次酸在0.05~10μg/ml浓度范围内线性关系良好;高、中、低浓度溶液的日内和日间RSD均小于3.4%;提取回收率为89.5%~107.9%,RSD为2.2%~4.6%。大鼠灌胃给予甘草次酸(50 mg/kg)后,血浆中甘草次酸的药动学参数分别为:cmax(28.48±6.90)μg/ml,tmax(3.00±1.07)h,t1/2(9.23±4.91)h,AUC0→∞(306.57±68.42)h·μg·ml-1,AUC0→t(290.93±68.36)h·μg·ml-1。
A HPLC method for the determination of glycyrrhetinic acid in rat plasma was established and its pharmacokinetics in rats were investigated. Symmetry ~ Shield C18 column with acetonitrile: 0.1% phosphoric acid as mobile phase, gradient elution, detection wavelength 254 nm. The linearity of glycyrrhetinic acid in the concentration range of 0.05 ~ 10μg / ml was good. The RSDs of high, medium and low concentration solutions were less than 3.4% in the day and day, and the recoveries were 89.5% -107.9% with RSD of 2.2% -4.6 %. The pharmacokinetic parameters of glycyrrhetinic acid in plasma after intragastric administration of glycyrrhetinic acid (50 mg / kg) were: cmax (28.48 ± 6.90) μg / ml and tmax (3.00 ± 1.07) h, t1 / 2 (9.23 ± 4.91) h, AUC0 → ∞ (306.57 ± 68.42) h · μg · ml-1, AUC0 → t (290.93 ± 68.36) h · μg · ml-1.