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[目的]比较具干细胞特性食管癌耐放射细胞株与其亲本基因表达水平的差异。[方法]Agilent4×44K人全基因组芯片检测具干细胞特性食管癌耐放射细胞株与其亲本细胞,GeneSpring GX 10.0.2数据分析挑选差异表达基因,进一步对差异表达基因进行GO分析和干细胞密切相关的信号通路分析,Real-time PCR验证芯片结果。[结果]两组细胞有明显不同的表达谱,差异表达基因多达2246个(fold change cut-off≥2)。在分子功能中,信号感应激活最高,占75.11%;在生物过程中,细胞进程、生物调节分别占35.94%和25.8%;在细胞组分方面,细胞、胞内区、跨膜区的变化比较显著。ALDH1基因表达发生了改变,其中ALDH1A3最显著。Hedgehog通路,PTCH1下调,而GLI2上调。BMP-TGFβ通路信号转导中,MMP3、MMP9上调,FOXL1、BMP4、TGFB2、TGFB3下调。Notch通路相关信号基因均表现为下调。Wnt通路信号转导变化比较复杂,其中WNT4、WNT8B、DKK2、CTNNB1改变超过2倍。Real-time PCR验证芯片结果可靠。[结论]具干细胞特性的食管癌放射抵抗细胞株与其亲本基因表达水平有明显差异。
[Objective] To compare the gene expression of esophageal cancer-resistant esophageal cancer cell lines with their parents. [Method] Agilent4 × 44K human whole genome microarray was used to detect esophageal cancer-resistant cell lines and their parental cells with the characteristics of stem cells. GeneSpring GX 10.0.2 data analysis was used to select the differentially expressed genes. GO analysis of the differentially expressed genes and the signals of closely related stem cells Pathway analysis, Real-time PCR validation chip results. [Results] The two groups of cells had obviously different expression profiles with as many as 2246 differentially expressed genes (fold change cut-off≥2). In the molecular function, the signal induction activation is the highest, accounting for 75.11%; in the biological process, the cell process, biological regulation accounted for 35.94% and 25.8% respectively; in the cell components, the changes in the cell, intracellular and transmembrane regions Significant. ALDH1 gene expression has changed, of which ALDH1A3 most significant. Hedgehog pathway, PTCH1 down, and GLI2 up. BMP-TGFβ pathway signaling, MMP3, MMP9 upregulation, FOXL1, BMP4, TGFB2, TGFB3 down. Notch pathway related signal genes showed down-regulation. Wnt pathway signaling changes more complicated, including WNT4, WNT8B, DKK2, CTNNB1 changes more than 2 times. Real-time PCR validation chip results are reliable. [Conclusion] There are significant differences in the gene expression of esophageal cancer cells and their parents with the characteristics of stem cells.