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利用游离小孢子培养技术对5个人工合成甘蓝型油菜品系进行了离体小孢子培养。从大田非控温控光条件下生长的供体植株第一朵花开后3～12d，取长度2．5～3．5mm的花蕾分离小鬼子，在NLN13液体培养基中进行薄层培养诱导成胚状体，然后用无激素的MS培养基诱导植株再生。对不同的供试材料、取蕾时期和培养基蔗糖浓度对小孢子胚胎发生能力的影响作了初步研究。供试的5个材料中有4个对培养有反应，但不同材料的小孢子胚胎发生能力有较大的差异。小孢子取样的时期以主花序第一朵花开后6d左右为宜，培养基蔗糖浓度以13％的效果最好。
In vitro microspore culture was carried out on five artificially synthesized Brassica napus lines using free microspore culture. From the first non-controlled temperature-controlled light field in Daejeon, 3 to 12 days after the first flower bloom, flower buds of 2.5-3.5 mm in length were used to separate small devils and induced into thin layer culture in NLN13 liquid medium Embryoid bodies were then induced to regenerate plants using hormone-free MS medium. The effects of different materials, budding period and medium sucrose concentration on microspore embryogenesis were studied. Four of the five materials tested responded to the culture, but the ability of different materials to produce microspore embryos was significantly different. Microspore sampling period of the first major flower inflorescence after about 6d is appropriate, the best concentration of 13% sucrose in culture medium.