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目的:探讨运动训练及心理应激对大鼠下丘脑神经肽Y(NPY)mRNA表达的影响。方法:将50只SD大鼠随机分为对照组、中等负荷运动组、急性心理应激组、慢性心理应激组、中等负荷运动+急性心理应激组、中等负荷运动+慢性心理应激组。实验期为10周。实验后对各组大鼠进行称重,并检测其下丘脑NPY mRNA表达。结果:(1)慢性心理应激大鼠体重显著低于对照组;急/慢性心理应激组大鼠下丘脑NPY mRNA水平均显著高于对照组。(2)中等负荷运动组大鼠体重显著高于对照组,且其下丘脑NPY mRNA水平显著高于对照组。(3)中等负荷运动+急/慢性心理应激组大鼠体重分别显著高于急/慢性心理应激组大鼠,其NPY mRNA水平与对照组相比无显著差异。结论:心理应激作为一种应激源,可能会引起大鼠神经肽分泌紊乱;中等负荷运动对提高机体抗应激能力有一定作用。
Objective: To investigate the effect of exercise training and psychological stress on the expression of neuropeptide Y (NPY) mRNA in rat hypothalamus. Methods: Fifty SD rats were randomly divided into control group, moderate load exercise group, acute psychological stress group, chronic psychological stress group, moderate load exercise + acute psychological stress group, medium load exercise + chronic psychological stress group . The experiment period is 10 weeks. After the experiment, the rats in each group were weighed and their hypothalamus NPY mRNA expression was measured. Results: (1) The body weight of chronic psychological stress rats was significantly lower than that of control rats. The level of NPY mRNA in hypothalamus in acute and chronic psychological stress rats was significantly higher than that in control rats. (2) The body weight of middle-weight exercise group was significantly higher than that of control group, and the level of NPY mRNA in hypothalamus was significantly higher than that of control group. (3) The body weight of rats in moderate stress exercise + acute / chronic psychological stress group were significantly higher than that in acute / chronic psychological stress group, and the NPY mRNA level had no significant difference compared with the control group. Conclusion: As a stress source, psychological stress may cause neuropeptide secretion disorder in rats. Moderate-stress exercise may play an important role in improving the anti-stress ability of the body.