论文部分内容阅读
背景:人类载脂蛋白H具有多态性,其与三酰甘油代谢及血小板功能有关,因而推测与冠状动脉粥样硬化性心脏病(冠心病)可能有一定的关系。目的:分析载脂蛋白H外显子8基因多态性与冠心病及脂代谢的相关性。设计:病例-对照观察。单位:山东省青岛市海慈医疗集团心内科。对象:选择青岛市海慈医疗集团心内科收治110例冠心病患者(经冠状动脉造影证实)为冠心病组,同期体检的100例健康志愿者为对照组。方法:抽取所有受试者外周静脉血2mL,采用聚合酶链式反应结合限制性片段长度多态分析方法,分析了2组的载脂蛋白H外显子8基因型;同时采用氧化酶法测定血脂。主要观察指标:①载脂蛋白H外显子8基因型多态性分析结果。②两组载脂蛋白H外显子8(Try316Ser)基因频率。③两组不同基因型者的血脂水平比较。结果:210例全部进入结果分析。①载脂蛋白H基因8号外显子存在G1025C(Try316Ser)多态,分别为GG和GC基因型,未发现CC型。②冠心病组GC基因型的频率为25.5%,C等位基因频率为0.13,显著高于对照组(10.0%,0.05,P<0.05)。③冠心病组GC基因型者的三酰甘油水平显著高于GG型和及对照组的各基因型[(1.38±0.24),(1.16±0.10),(1.09±0.78),(1.12±0.76)mmol/L,P<0.05];冠心病组低密度脂蛋白胆固醇高于对照组(P<0.05)。结论:载脂蛋白H外显子8基因多态性与冠心病、三酰甘油相关。
BACKGROUND: Human apolipoprotein H is polymorphic and is associated with triglyceride metabolism and platelet function. Therefore, it is speculated that coronary artery disease may be related to coronary heart disease (CAD). Objective: To analyze the association of apolipoprotein H exon 8 polymorphism with coronary heart disease and lipid metabolism. Design: Case-control observation. Unit: Qingdao City, Shandong Province sea-Medical Group of Cardiology. PARTICIPANTS: One hundred and ten patients with coronary heart disease (confirmed by coronary angiography) were selected as the CHD group in the Department of Cardiology, Haicheng Medical Group, and 100 healthy volunteers in the same period were selected as the control group. Methods: Peripheral venous blood (2mL) was drawn from all the subjects. The genotypes of apolipoprotein H exon 8 were analyzed by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The oxidative enzyme assay Blood lipids. MAIN OUTCOME MEASURES: ① Apolipoprotein H exon 8 genotype polymorphism analysis results. ② two groups of apolipoprotein H exon 8 (Try316Ser) gene frequency. ③ two groups of different genotypes of blood lipid levels compared. Results: 210 cases all entered the result analysis. ① Exon 8 of the apolipoprotein H gene has G1025C (Try316Ser) polymorphism, which are GG and GC genotypes, respectively. No CC genotype was found. The frequency of GC genotype was 25.5% in coronary heart disease group and 0.13 in C allele, which was significantly higher than that in control group (10.0%, 0.05, P <0.05). (3) The triglyceride levels of GC genotype in CHD group were significantly higher than those in GG group and control group [(1.38 ± 0.24), (1.16 ± 0.10), (1.09 ± 0.78), (1.12 ± 0.76) mmol / L, P <0.05]. The level of LDL-C in CHD group was higher than that in control group (P <0.05). Conclusion: Apolipoprotein H exon 8 gene polymorphism is associated with coronary heart disease and triglyceride.