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目的 研究胆囊癌组织及相应胆汁中的细菌菌谱，以探讨胆囊癌的发生与细菌感染的关系。方法 采用聚合酶链反应（ＰＣＲ） ，对３７ 例胆囊癌组织标本及９ 例相应胆汁中的细菌ＤＮＡ 片段进行分子生物学扩增。结果 ３７ 例胆囊癌组织标本中细菌ＤＮＡ 片段检出率为７８－３７％ （２９／３７），９ 例相应胆汁中细菌ＤＮＡ检出率为７７－８％ （７／９）。结论 胆囊粘膜癌变可能与厌氧菌尤其是产气荚膜梭菌感染存在联系，厌氧菌与需氧菌协同作用，长期刺激胆囊粘膜可能是引起癌变的主要因素。
Objective To study the bacterial spectrum of gallbladder carcinoma and corresponding bile in order to investigate the relationship between the occurrence of gallbladder carcinoma and bacterial infection. Methods Polymerase chain reaction (PCR) was used to perform molecular biological amplification on 37 gallbladder cancer tissue samples and 9 corresponding bile bacterial DNA fragments. Results The detection rate of bacterial DNA fragments in 78 cases of gallbladder carcinoma was 78-37% (29/37). The corresponding bacterial detection rate in 9 cases was 77-8% (7/9). Conclusion Carcinogenesis of gallbladder mucosa may be associated with anaerobic bacteria, especially Clostridium perfringens. Anaerobic bacteria and aerobic bacteria may act synergistically. Prolonged stimulation of gallbladder mucosa may be the main cause of canceration.