本实验以人造釉器为材料,对碱性磷酸酶等三种酶进行了组织化学定位,并注意到在硬组织形成阶段,中间层细胞具有较强的碱性磷酸酶和无机焦磷酸酶的活性。根据这些细胞的分布,推测其具有分解过滤无机焦磷酸盐的作用以促进钙化。造釉细胞中存在特异性钙激活三磷酸腺苷酶,为钙离子是通过造釉细胞体进入釉基质的论点提供了依据。 In this experiment, artificial enamel was used as a material to histochemically locate three enzymes such as alkaline phosphatase. It was also noted that in the stage of hard tissue formation, the middle layer cells have strong alkaline phosphatase and inorganic pyrophosphatase active. Based on the distribution of these cells, it is presumed that they have the effect of decomposing and filtering inorganic pyrophosphates to promote calcification. The presence of a specific calcium-activated adenosine triphosphate enzyme in ameloblasts provides the basis for the notion that calcium ions enter the enamel matrix through ameloblasts.