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目的:建立尿液中丁丙诺啡GC/MS分析方法。方法:在含丁丙诺啡的尿液中,加入β-葡萄糖醛酸酶溶液,50℃水解2h,加入内标长春西汀,加pH7缓冲溶液,用三氯甲烷提取,提取物经N,O-双(三甲基硅烷基)三氟乙酰胺衍生化后进行GC/MS分析。GC/MS条件:HP-5MS(30m×0.25mm×0.25μm)石英毛细管柱,柱温:初温240℃保持2min,以20℃.min-1速率升至280℃保持18min。结果:线性范围为0.5～100μg·L-1,检出限为0.2μg·L-1。结论:该方法灵敏度高,可用于涉毒案件尿液中丁丙诺啡的分析。
Objective: To establish a method for the analysis of buprenorphine in urine by GC / MS. Methods: In the urine containing buprenorphine, β-glucuronidase solution was added and hydrolyzed at 50 ℃ for 2h. The internal standard vinpocetine was added, and the buffer solution of pH7 was added. The extract was extracted with chloroform. The extract was separated by N, O-bis (trimethylsilyl) trifluoroacetamide was derivatized for GC / MS analysis. GC / MS conditions: HP-5MS (30 m × 0.25 mm × 0.25 μm) quartz capillary column. Column temperature: The initial temperature was maintained at 240 ° C. for 2 min and the temperature was raised to 280 ° C. at 20 ° C.min-1 for 18 min. Results: The linear range was 0.5 ~ 100μg · L-1, the detection limit was 0.2μg · L-1. Conclusion: The method is sensitive and can be used for the analysis of buprenorphine in the urine of drug-related cases.