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目的探讨DICER基因微小RNA结合区域单核苷酸多态性(single nucleotide polymorphism,SNP)与有氧运动能力表型的关联。方法选择178名大学新生进行18周的长跑训练,测定训练前后最大摄氧量绝对值(VO2max)、摄氧量相对值(rVO2max);进行跑节省化实验测定12 km/h跑速下的RE各项指标;同时测定训练前后受试者心室结构和功能相关指标。采用PCR-RFLP基因分型技术检测DICER基因rs1057035位点多态性。采用荧光素酶报告基因系统对rs1057035位点进行功能研究。结果 DICER基因rs1057035多态位点与VO2max、RE、心室结构和功能相关指标的初始值没有关联,但是与VO2max、心室结构和功能相关指标的训练敏感性有关联。长跑训练后,TC+CC基因型受试者VO2max和rVO2max变化量显著高于TT基因型者;TC+CC基因型受试者心室结构指标ESD、IVSS、PWD、LVM和LVMI变化量显著高于TT基因型者;TC+CC基因型受试者心室功能指标SV、EF变化量显著高于TT基因型者,而T变化量则显著低于TT基因型者;经协方差分析,这些差异均具有统计学意义(P<0.05)。另外,基因功能研究显示,与rs1057035位点T基因型相比,C基因型显著降低DICER基因的表达活性。结论 DICER基因rs1057035多态位点与研究对象长跑训练后有氧运动能力表型存在着关联,长跑训练后TC+CC基因型者VO2max以及部分心室结构和功能改变显著高于TT基因型者,该位点多态性可以成为人群训练敏感性的分子遗传学标记。
Objective To explore the association of single nucleotide polymorphism (SNP) with the aerobic capacity phenotype in DICER gene microRNA binding region. Methods 178 college freshmen were selected for 18-week long-distance running training. The absolute value of maximum oxygen uptake (VO2max) and the relative oxygen uptake (rVO2max) before and after training were measured. The run-saving experiment was performed to determine the running speed of 12 km / h Under the RE indicators; at the same time before and after training to determine the subject’s ventricular structure and function-related indicators. Polymorphism of rs1057035 in DICER gene was detected by PCR-RFLP genotyping. The luciferase reporter gene system was used to study the function of rs1057035. Results The rs1057035 polymorphism site of DICER gene was not associated with the initial values of O2VO2max, RE, ventricular structure and function-related indices, but was associated with 练VO2max, training sensitivity of ventricular structure and function-related parameters. After long-distance training, the changes of VO2max and rVO2max in subjects with TC + CC genotype were significantly higher than those with TT genotype; The changes of ventricular structural parameters ESD, IVSS, PWD, LVM and LVMI in subjects with TC + CC genotype Significantly higher than the TT genotype; TC + CC genotype subjects ventricular function SV, EF changes were significantly higher than the TT genotype, and T changes were significantly lower than the TT genotype; by covariance analysis, These differences were statistically significant (P <0.05). In addition, gene function studies showed that genotype C significantly decreased the expression of DICER gene compared with the rs1057035 T genotype. Conclusion There is a relationship between rs1057035 polymorphism of DICER gene and aerobic capacity after long-distance running training in TC + CC genotype. The VO2max and some changes of ventricular structure and function in TC + CC genotype were significantly higher than that of TT genotype, Polymorphism at this locus can be a molecular genetic marker of crowd training sensitivity.