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目的探讨慢性乙型肝炎患者肝组织中Foxp3表达及定位情况,并分析其与肝组织病理及血清学相关性。方法慢性乙型肝炎患者119例,对肝组织进行病理炎症活动度分级(G0~G4组)和纤维化分期(S1~S4组)。免疫组织化学法测定肝组织中Foxp3表达,并进行半定量分析。结果肝组织汇管区、小叶内Foxp3阳性表达G1~G4组均高于G0组(P均<0.05),肝组织汇管区Foxp3阳性表达量与炎症活动度呈正相关(r=0.561,P<0.01),但小叶区Foxp3各组阳性表达与炎症活动度无相关性(r=0.133,P>0.05)。Foxp3表达与不同肝纤维化分期、血清ALT水平及HBV DNA载量之间均无相关性(P均>0.05)。结论汇管区Foxp3的表达与病理炎症活动度具有相关性,迁延不愈的免疫损伤可能与机体缺少足够的调节性T细胞抑制有关。
Objective To investigate the expression and localization of Foxp3 in the liver tissue of patients with chronic hepatitis B and analyze its correlation with liver histopathology and serology. Methods A total of 119 patients with chronic hepatitis B were enrolled in this study. Pathological changes of inflammatory activity (G0-G4 group) and fibrosis stage (S1-S4 group) were observed. Immunohistochemistry was used to detect Foxp3 expression in liver tissue and semi-quantitative analysis. Results The expression of Foxp3 in hepatic tissue and lobular tissue was higher in G1 ~ G4 group than in G0 group (all P <0.05). The positive expression of Foxp3 in hepatic tissue was positively correlated with inflammatory activity (r = 0.561, P <0.01) , But there was no correlation between the positive expression of Foxp3 and the activity of inflammation in the lobular area (r = 0.133, P> 0.05). There was no correlation between Foxp3 expression and different stage of liver fibrosis, serum ALT level and HBV DNA load (all P> 0.05). Conclusion The expression of Foxp3 in portal area is correlated with the degree of pathological inflammatory activity. The delayed immune damage may be related to the lack of sufficient regulatory T cell suppression in the body.