论文部分内容阅读
目的 通过辐射人喉鳞癌细胞株Hep- 2获得辐射耐受细胞株Hep- 2R ,建立一个放射敏感性研究的对比模型。方法 用γ线反复照射Hep- 2细胞,建立辐射耐受细胞Hep- 2R。成克隆实验法测定两株细胞不同剂量照射后的细胞存活分数,拟合细胞存活曲线比较两种细胞株的放射生物学参数。光镜、电镜、活细胞计数、染色体分析及流式细胞仪周期分布比较其生物学差异。结果 经7个月辐射诱导得到了一个放射敏感性不同于亲本细胞株的Hep -2R细胞株,并已稳定传30代以上且辐射耐受性能稳定。其SF2 =0 .6 80、D0 =3.2 4Gy、Dq=1.90Gy、N =1.80 ,而Hep 2细胞SF2 =0 .4 15、D0=2 .0 6Gy、Dq=1.0 1Gy、N =1.6 4。Hep -2R细胞形态及染色体数目发生了改变,群体倍增时间较亲本细胞延长。细胞周期分析显示G1期细胞增多,S、G2 期细胞减少。结论 通过辐射诱导可以从Hep -2细胞株得到辐射耐受细胞株Hep -2R ,这种相同背景、不同放射敏感细胞株为进一步研究放射敏感性的分子机制提供了一个良好对比模型。
OBJECTIVE: To establish a radiosensitive study model of radiation-resistant cell line Hep-2R by radiating human laryngeal squamous carcinoma cell line Hep-2. Methods Hep-2 cells were irradiated with γ-ray repeatedly to establish Hep-2R cells. The clonogenic assay was used to determine the cell viability of the two cells after irradiation at different doses. The survival curves of the two cell lines were fitted to compare the radiobiological parameters. Light microscopy, electron microscopy, viable cell count, chromosome analysis and cycle distribution of flow cytometry to compare their biological differences. Results Hep-2R cell line with different radiosensitivity from the parental cell line was induced by radiation for 7 months and was stable for more than 30 passages and its radiation tolerance was stable. SF2 = 0.680, D0 = 3.2 4Gy, Dq = 1.90Gy, N = 1.80, while Hep 2 cells SF2 = 0.415, D0 = 2.06Gy, Dq = 1.01Gy, and N = 1.6 4. Hep-2R cell morphology and chromosome number has changed, population doubling time longer than the parental cells. Cell cycle analysis showed that G1 phase cells increased, S, G2 phase cells decreased. Conclusion Radiation-tolerant cell line Hep-2R can be obtained from Hep-2 cells by radiation. Different radiosensitive cell lines provide a good contrast model for further studying the molecular mechanism of radiosensitivity.