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株高是典型的数量性状,易受遗传背景的干扰和环境因素的影响,利用单片段代换系(single segment substitution lines,SSSLs)能减少遗传背景的干扰。以85个单片段代换系为材料,其受体亲本为籼稻广陆矮4号(Oryzasativassp.in-dica),供体亲本为粳稻日本晴(Oryza sativa ssp.japonica)。通过单因素方差分析和Dunnett’s多重比较,分析单片段代换系与受体亲本之间株高的差异,对代换片段上的株高QTL进行鉴定。以P≤0.001为阈值共检测到24个株高QTLs,分别分布于除第10染色体外的其它11条染色体上,其中3个QTLs的加性效应表现为减效作用,另外21个株高QTLs的加性效应表现为增效作用。QTLs加性效应变化范围为-6.5-31.74,加性效应百分率的变化范围为-8.81%-41.96%。该研究对进一步发掘和利用新的矮秆或半矮秆基因资源具有重要意义。
Plant height is a typical quantitative trait susceptible to genetic background disturbances and environmental factors, and the use of single segment substitution lines (SSSLs) can reduce genetic background interference. The 85 single-segment substitution lines were used as materials, and the recipient parent was Oryza sativa subsp. In-dica and the donor was Oryza sativa ssp.japonica. Through the one-way analysis of variance and Dunnett’s multiple comparisons, the differences of plant height between the single-segment substitution lines and the recipient parents were analyzed, and the QTLs for plant height in the substitution segments were identified. Twenty-four plant height QTLs were detected with P≤0.001 as the threshold value, respectively, distributed in 11 other chromosomes except chromosome 10, of which 3 QTLs showed additive effects and the other 21 QTLs The additive effect of the performance of the synergistic effect. The range of additive effect of QTLs was -6.5-31.74, and the percentage of additive effect ranged from -8.81% to 41.96%. This research is of great significance for further exploration and utilization of new dwarf or semi-dwarf gene resources.