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目的:观察青藤碱对大鼠肝脏缺血再灌注损伤的影响,探讨其保护大鼠肝脏缺血再灌注损伤作用的机制。方法:通过建立大鼠全肝缺血再灌注损伤模型,应用硝酸酶还原法测定肝脏缺血再灌注后60min血清NO水平变化;测定再灌注60min后肝组织内MDA和SOD含量变化;再灌注60min取肝组织完成肝组织显微结构的观察。结果:肝脏缺血再灌注损伤后血清NO水平降低;青藤碱能提高再灌注后血清NO水平,且能改善肝脏缺血再灌注损伤的微循环,减轻肝细胞内超微结构的损害程度。结论:青藤碱对大鼠肝脏缺血再灌注损伤有保护作用,其主要作用机制是清除氧自由基和改善微循环。
Objective: To observe the effect of sinomenine on hepatic ischemia-reperfusion injury in rats and its mechanism of protecting rat liver from ischemia-reperfusion injury. Methods: The model of total hepatic ischemia-reperfusion injury in rats was established. The level of serum NO was measured 60 min after ischemia-reperfusion in liver by nitratase reduction. The content of MDA and SOD in liver tissue were measured 60 min after reperfusion. Liver tissue was taken to observe the microstructure of liver tissue. Results: Serum NO level decreased after ischemia-reperfusion injury in liver. Sinomenine could increase the serum NO level after reperfusion and improve the microcirculation of hepatic ischemia-reperfusion injury and alleviate the damage of hepatic ultrastructure. CONCLUSION: Sinomenine has a protective effect on hepatic ischemia-reperfusion injury in rats. Its main mechanism is to scavenge oxygen free radicals and improve microcirculation.