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三十年代末低温技术用于红内期疟原虫保种,20余年后得知一定浓度的甘油或二甲基亚砜是良好的冰冻保护剂。Strome等认为液氮可无限期地保存红内期疟原虫的感染力。我们自1973年以来的观察表明,红内期疟原虫的低温保存法确实可靠。从60年代开始,国外开始探索子孢子的低温保存法。有些疟原虫子孢子的低温保存仍须另加同种动物血清或血浆,操作繁琐,且最佳冷冻条件尚在研究中。我们自1980年10月先用鸡疟原虫初试成功后,又对食蟹猴疟原虫进行试验,建立了不加任何保护剂的
The late thirties of low temperature technology for red-phase maintenance of Plasmodium, 20 years later that a certain concentration of glycerol or dimethyl sulfoxide is a good cryoprotectant. Strome and others believe that liquid nitrogen can indefinitely preserve the infectivity of red-phase Plasmodium. Our observations since 1973 show that cryopreservation of Plasmodium in the red is indeed reliable. From the 1960s, foreign countries began to explore the sporozoites preservation method. Some Plasmodium sporozoites cryogenic preservation still need to add the same animal serum or plasma, cumbersome operation, and the optimal freezing conditions are still under study. After the first test of Plasmodium falciparum was successfully carried out in October 1980, we tested the Plasmodium cynomolgus malaria parasite to establish a non-protective