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目的探讨黄芪甲苷对大鼠脑缺血再灌注后血脑屏障的保护作用及其机制。方法将SD大鼠72只随机等分为4组:假手术组、生理盐水对照组、小剂量黄芪甲苷治疗组(10 mg/kg)和大剂量黄芪甲苷治疗组(20 mg/kg),采用分光光度计法、酶联免疫吸附法及免疫组化法分别检测各组大鼠脑组织伊文氏蓝、IL-1β含量及MMP-9蛋白的表达水平。结果与假手术组相比,生理盐水对照组脑组织伊文氏蓝含量明显增多、IL-1β含量显著增高、MMP-9蛋白的表达明显增强(P<0.01);与生理盐水对照组相比,小剂量黄芪甲苷治疗组及大剂量黄芪甲苷治疗组脑组织伊文氏蓝含量均显著减少、IL-1β含量明显降低、MMP-9蛋白表达明显减弱(P<0.05);小剂量黄芪甲苷治疗组与大剂量黄芪甲苷治疗组相比,伊文氏蓝、IL-1β含量及MMP-9蛋白表达无显著差异(P>0.05)。结论黄芪甲苷对脑缺血再灌注后血脑屏障具有保护作用,这可能与其下调IL-1β含量、抑制MMP-9蛋白的表达有关。
Objective To investigate the protective effect of astragaloside on blood-brain barrier (BBB) after cerebral ischemia-reperfusion in rats and its mechanism. Methods Seventy-two SD rats were randomly divided into 4 groups: sham operation group, saline control group, small dose of Astragaloside treatment group (10 mg / kg) and high dose Astragaloside treatment group (20 mg / kg) . The content of IL-1β and the expression of MMP-9 in brain tissue of rats in each group were detected by spectrophotometer, enzyme-linked immunosorbent assay and immunohistochemistry. Results Compared with the sham-operated group, the content of IL-1β in brain tissue of normal saline group was significantly increased, and the expression of MMP-9 protein was significantly increased (P <0.01). Compared with saline control group, Low-dose Astragaloside treatment group and high-dose Astragaloside treatment group, brain tissue Evans blue content were significantly reduced, IL-1β content was significantly reduced, MMP-9 protein was significantly decreased (P <0.05); low-dose Astragaloside IV Compared with the high-dose Astragaloside treatment group, the levels of IL-1β and MMP-9 in the treatment group were not significantly different (P> 0.05). Conclusion Astragaloside has a protective effect on the blood-brain barrier after cerebral ischemia-reperfusion, which may be related to down-regulating the content of IL-1β and inhibiting the expression of MMP-9.