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目的:建立同时测定葛根芩连微丸中葛根素、甘草苷、黄芩苷、盐酸小檗碱含量的方法。方法:采用RP-HPLC方法,色谱柱为Kromasil-C18(4.6 mm×250 mm,5μm),流动相选用甲醇-磷酸盐缓冲液系统,梯度系统,流速1.0 mL.min-1,检测波长275 nm。结果:4个成分的方法学考察均合格,阴性供试品无干扰。结论:该方法方便、准确、专属性强,可作为葛根芩连微丸质量控制的进一步补充。
Objective: To establish a method for simultaneous determination of puerarin, glycyrrhizin, baicalin and berberine hydrochloride in Gegen Qinlianlian pellet. Methods: The mobile phase was Kromasil-C18 (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of methanol-phosphate buffer system with gradient system at a flow rate of 1.0 mL · min-1. The detection wavelength was 275 nm . Results: The four components of the methodological study were qualified, negative for the test without interference. Conclusion: The method is convenient, accurate and specific and can be used as a supplement to the quality control of Gegen Qinlian pellets.