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目的:探讨姜黄素衍生物T63在鼻咽癌CNE-2和CNE-2R细胞中的抗癌作用。方法:采用MTT法、集落形成实验和流式细胞术检测单独放疗组、药物处理组和放疗药物联合作用组对CNE-2和CNE-2R细胞增殖能力、细胞凋亡和细胞周期的影响,并比较其差异。结果:T63可有效抑制CNE-2和CNE-2R细胞的活性和增殖;T63或电离辐射(IR)均可单独诱导CNE-2和CNE-2R细胞的凋亡和G2/M期阻滞;与T63或IR单独作用组比,T63与IR联合作用诱导细胞凋亡和G2/M期阻滞更明显(P<0.05)。结论:T63通过增加放疗诱导的细胞凋亡和G2/M期阻滞而逆转CNE-2R细胞辐射抗性,提示T63具有放疗增敏的潜在价值。
Objective: To investigate the anticancer effect of curcumin derivative T63 in nasopharyngeal carcinoma CNE-2 and CNE-2R cells. Methods: MTT assay, colony formation assay and flow cytometry were used to detect the effects of radiotherapy, drug treatment and radiotherapy on the proliferation, apoptosis and cell cycle of CNE-2 and CNE-2R cells. Compare their differences. Results: T63 could effectively inhibit the activity and proliferation of CNE-2 and CNE-2R cells. Both T63 and ionizing radiation (IR) induced CNE-2 and CNE-2R cell apoptosis and G2 / M arrest alone. Compared with T63 or IR alone, the combination of T63 and IR induced cell apoptosis and G2 / M arrest more significantly (P <0.05). CONCLUSION: T63 reverses the radiation resistance of CNE-2R cells by increasing radiotherapy-induced apoptosis and G2 / M arrest, suggesting that T63 has the potential value of radiosensitization.