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目的:观察六味地黄汤对发育迟缓仔鼠认知功能及脑组织NGF、EGF、b FGF表达影响,阐述六味地黄汤补肾健脑的机制。方法:采用孕鼠被动吸烟复制仔鼠发育迟缓模型,于自然分娩后喂养仔鼠1个月,进行学习记忆能力的检测,采用免疫组化法及RT-q PCR检测仔鼠脑内NGF、EGF、b FGF的表达水平。结果:1造模后,与正常组相比,动物逃逸潜伏期延长,垮台次数减少(P<0.05),药物治疗后,与模型组相比,逃逸潜伏期缩短,垮台次数增多,差异具有统计学意义(P<0.05)。2正常仔鼠脑内NGF、EGF、b FGF基因和蛋白表达大致相同,造模后,NGF、EGF、b FGF表达减少,与正常组相比,差异具有统计学意义(P<0.01或P<0.05),药物治疗后,NGF、EGF、b FGF表达明显增多,与模型组相比,差异具有统计学意义(P<0.01或P<0.05)。结论:孕期被动吸烟可以导致仔鼠学习记忆能力降低以及脑组织NGF、EGF、b FGF表达下降,六味地黄汤能显著提高脑组织内NGF、EGF、b FGF表达水平,从而提高仔鼠的学习记忆能力可能是其补肾健脑的机制。
Objective: To observe the effect of Liuwei Dihuang decoction on the cognitive function and the expression of NGF, EGF and b FGF in brain of developing mice, and to elucidate the mechanism of Liuwei Dihuang decoction for invigorating kidney and brain. Methods: The pregnant rats were reproduced by passive smoking. The offspring model was fed to the offsprings after natural delivery for one month. The learning and memory ability of the offspring mice was detected by immunohistochemistry and RT-q PCR. The levels of NGF and EGF , B FGF expression levels. Compared with the normal group, the escape latency and the number of the downtime of the animals were decreased (P <0.05). Compared with the model group, the escape latency and the number of the downtime increased significantly, the difference was statistically significant (P <0.05). The expression of NGF, EGF and b FGF in normal rats were similar, and the expression of NGF, EGF and b FGF decreased after modeling, compared with the normal group, the difference was statistically significant (P <0.01 or P < 0.05). After drug treatment, the expression of NGF, EGF and b FGF were significantly increased compared with the model group, the difference was statistically significant (P <0.01 or P <0.05). Conclusion: Passive smoking during pregnancy can lead to decreased ability of learning and memory, decreased expression of NGF, EGF and b FGF in brain, and Liuweidihuang Decoction can significantly increase the expression of NGF, EGF and b FGF in brain tissue, thereby improving learning and memory of pups Ability may be its mechanism of kidney and brain.