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1990年1～5月,焦作地区博爱县、沁阳市、中站区出现了大批腹泻病人,经病原学证实是由成人轮状病毒引起。现将调查结果报告如下。调查对象凡近期内发生的腹泻病人由县、乡、村防疫人员进行普查、登记。确定4个发病较多的村庄和1个电厂的所有居民为调查对象。对具有流行性腹泻临床特点的病例(包括痊愈者)由市、县防疫人员逐项填写统一的个案调查表。标本搜集和病原学检查采集发病2天内腹泻病人新鲜稀便10ml于瓶内,-20℃冻存待检。同肘将腹泻病人的鲜便接种于Cang-Blair 运送培养基中,当天送检。按照河南省卫生防疫站《急性细菌性腹泻常规实验室诊断技术》分别进行沙门氏菌、志贺氏菌、埃希氏菌、耶氏菌、气单胞、邻单胞、弧菌、空肠弯曲菌和小肠结肠炎耶氏菌的分离和鉴定;轮状病毒用核酸电泳法检测。诊断血清均为兰州生物制品研究所生产。另采集疫区污水、生活用水进行细菌学检测。
From January to May 1990, a large number of patients with diarrhea appeared in Piao County, Qinyang City, and Zhongzhan District in Jiaozuo. The etiology confirmed that they were caused by adult rotavirus. The survey results are reported below. Where the investigation of patients with diarrhea occurred in the near future by the county, township and village epidemic prevention staff to conduct census and registration. Identify all 4 inhabitants in the more affected villages and 1 power plant as respondents. Cases of epidemic diarrhea with clinical features (including those who are cured) by city and county epidemic prevention staff itemized a single case-by-case questionnaire. Specimen collection and etiological examination Acquired fresh diarrhea 10ml of diarrhea patients in the bottle within 2 days, frozen at -20 ℃ to be seized. The same elbow diarrhea patients inoculated in Cang-Blair transport medium, the same day submission. Salmonella, Shigella, Escherichia, Yersuvial, gas cell, neighbors, Vibrio, Campylobacter jejuni, and Campylobacter jejuni were performed according to the “routine laboratory diagnostic technique for acute bacterial diarrhea” Isolation and identification of Yersinia enterocolitica; Rotavirus was detected by nucleic acid electrophoresis. Diagnostic serum Lanzhou Institute of Biological Products are produced. Another collection of the affected area sewage, domestic water for bacteriological testing.