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通过溶液培养试验,研究了2个箭筈豌豆(Vicia sativa L.)品种(镉耐性品种L3,镉敏感品种ZM)镉胁迫下含水量和渗透调节物质含量的变化,以及2个品种对失水胁迫的耐性。结果表明:25μmol/L镉处理14d,L3和ZM根中含水量分别较对照降低1.27%和4.66%,地上部含水量分别较对照降低4.06%和5.46%,ZM失水更明显;镉胁迫下,ZM和L3根中总氨基酸、脯氨酸和还原糖含量以及茎中总氨基酸含量都较对照显著升高,ZM较L3更明显;ZM叶中脯氨酸和还原糖含量也较对照显著升高,L3叶中二者较对照升高不显著;同时,ZM较L3有更强的对失水胁迫的耐性。因此,镉胁迫下箭筈豌豆渗透调节物质的积累是对镉胁迫引起的失水胁迫的积极响应;箭筈豌豆对失水胁迫的耐性不是决定其镉耐性的主要方面。
The changes of water content and osmoregulation substances in two Vicia sativa L. cultivars (Cd-tolerant cultivar L3 and ZM-sensitive cultivar ZM) under cadmium stress were studied by solution culture test, and the effects of two cultivars on water loss Stress tolerance. The results showed that after 25 μmol / L cadmium treatment for 14 d, the water contents of L3 and ZM roots decreased by 1.27% and 4.66% respectively compared with the control, while the aboveground water content decreased 4.06% and 5.46% respectively compared with the control and the ZM water loss was more obvious. Under cadmium stress ZM and L3, the content of total amino acids, proline and reducing sugar and the content of total amino acids in stems were significantly higher than that of the control, ZM was more obvious than that of L3; the content of proline and reducing sugar in ZM leaves was also significantly increased High and L3 leaves in the two compared with the control did not increase significantly; the same time, ZM than L3 have a greater resistance to water stress. Therefore, the accumulation of osmoregulation substances under Cd stress was a positive response to water stress induced by cadmium stress. Tolerance to water deficit was not the main factor determining cadmium tolerance.