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为研究检测人肿瘤组织细胞多药耐药基因表达产物，采用间接标记法，用鼠抗人Ｐ１７０单克隆抗体，二抗为ＦＩＴＣ标记兔抗鼠Ｉｇ，应用流式细胞仪检测了２９例新鲜实体肿瘤组织和一株多药耐药的Ｋ５６２细胞株。结果显示：１８例肿瘤细胞表达Ｐ１７０，其阳性率为６２．１％，１１例未检出表达，其阴性率为３７．９％；９９．９％Ｋ５６２细胞表达Ｐ１７０。在１８例Ｐ１７０阳性表达的样本中，有１６例阳性表达百分比低于３０％；只有２例高于３０％。提示大多数肿瘤组织细胞表达Ｐ１７０阳性率高，但表达Ｐ１７０肿瘤细胞表达百分比低。检测肿瘤组织的Ｐ１７０表达能为临床治疗提供一个可靠的参考指标。
In order to study the detection of multidrug resistance gene expression products in human tumor cells, indirect labeling was used. Mouse anti-human P170 monoclonal antibody and secondary antibody FITC were used to label rabbit anti-mouse Ig. 29 fresh entities were detected by flow cytometry. Tumor tissue and a multidrug resistant K562 cell line. The results showed that: 18 cases of tumor cells expressed P170, the positive rate was 62.1%, 11 cases were not detected, the negative rate was 37.9%; 99.9% K562 cells express P170. Of the 18 samples with positive P170 expression, 16 were positive for less than 30%; only 2 were higher than 30%. It is suggested that the majority of tumor cells express P170 with high positive rate, but express P170 tumor cells with low expression percentage. Detecting P170 expression in tumor tissue can provide a reliable reference for clinical treatment.