本文介绍用免疫荧光试验检测人外周血 B 淋巴细胞的S_mI_gG~+细胞率和帽状形成百分率,观察不同剂量~(60)Co γ射线体外照射对淋巴细胞帽状反应的影响,并用松胞素 B 或秋水仙素处理淋巴细 胞,与单纯照射或药物加照射后的影响相比较,以探讨辐射损伤的可能机理。结果表明,电离辐射对淋巴细胞的帽状反应具有抑制作用;辐射对帽状反应的抑制作用,除电离辐射直接引起质膜损伤外,还可能与微丝、微管功能受损有关。 This article describes the use of immunofluorescence assay to detect human peripheral blood B lymphocytes S_mI_gG ~ + cell rate and the percentage of cap formation, observe the effect of different doses of ~ (60) Co γ-rays in vitro on the lymphocyte cap reaction, and use cytosine B or colchicine treatment of lymphocytes, compared with the effects of irradiation alone or drug plus irradiation, to explore the possible mechanism of radiation damage. The results showed that ionizing radiation had an inhibitory effect on the cap-like reaction of lymphocytes; the inhibitory effect of radiation on the cap-like reaction, in addition to plasma membrane damage caused by ionizing radiation, may also be associated with impaired microfilament and microtubule function.