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本文以素心建兰(金丝凤尾素和铁骨素)的茎尖或腋芽为外植体,分别培养在修改的BMS或BMS+10％椰乳(CM)+5mg/l萘乙酸(NAA)的琼脂培养基上,经三个月诱导发生了原球茎,将原球茎切割,培养在相同成分的液体培养基中,继代增殖。原球茎转入BMS+10％CM液体培养基中,连续照光,在其顶端分化芽,由芽基部分化根,形成小植株,这样建立了快速无性繁殖系。将小植株转入1/2BMS琼脂培养基上,每天 8小时照光。幼苗生长发育,由其腋芽分化发育为花芽,然后在花芽基部形成根,花葶挺拔而出、花蕾开启。试管育兰一年四季能得到开花植株。对兰花生长发育及花芽形成规律进行了讨论。实验结果表明,盆栽兰花;每株的腋芽可以发育成只长叶不开花的个体。当腋芽顶端的分生组织分化为花芽,花葶挺拔而出。开花的个体,叶片不发育,而成为常见的苞叶及鞘。
In this study, shoot tips or axillary buds of Pleurotus citrinopileatus were cultured in modified BMS or BMS + 10% coconut milk (CM) + 5mg / l NAA ) Agar medium, the protocorm induced after three months, the protocorm cut, cultured in the same composition of the liquid medium, the proliferation of the next generation. Protocorms were transferred to BMS + 10% CM liquid medium and continuously irradiated with light to differentiate buds at their apical ends and to differentiate their roots from bud bases to form plantlets, thus establishing a rapid clonal propagation line. The plants were transferred to 1 / 2BMS agar medium, 8 hours a day light. Seedling growth and development, from the axillary bud differentiation into flower buds, and then form the base of the flower buds, scapes tall and straight, flower buds open. Test tube Yu Lan all year round to get flowering plants. The orchid growth and flower bud formation were discussed. The experimental results show that potted orchids; axillary buds per plant can develop into only long-leafed individuals without flowering. When the top of the axillary buds differentiate into flower buds, scapes tall and straight out. Flowering individuals, leaves are not developed, and become a common bract and sheath.