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为加深和丰富对我国最为重要的经济虾类之一日本对虾(Penaeus japonicus)遗传多样性的认识,分析了日本对虾一个野生地理种群(台湾海峡北部)及其移植到北方水域进行繁育的养殖群体各23个个体的随机扩增DNA多态性。实验选取OPK组20个10 bp随机引物中的17个产生效果稳定的扩增结果用于群体遗传多样性分析,在两个群体中共检测出155个位点,野生种群和养殖群体的多态比例分别为85.14％和78.62％.用经修正的Shannon表型多样性指数量化两个群体的遗传多态度,野生种群和养殖群体的遗传多态度分别为0.214和0.201;两群体的遗传相似度为94.20％,遗传距离为0.058.
In order to deepen and enrich the understanding of the genetic diversity of Penaeus japonicus, one of the most important economic shrimp species in our country, the analysis of a wild geographic population of shrimp (northern Taiwan Strait) and its breeding population transplanted to northern waters for breeding Random amplified DNA polymorphism for each of 23 individuals. In the experiment, 17 out of 20 10 bp random primers in OPK group were used to generate stable amplification results for population genetic diversity analysis. A total of 155 loci were detected in the two populations. The polymorphic proportions of wild population and cultured population Respectively, 85.14% and 78.62% respectively.The genetic diversity of two populations was quantified using the modified Shannon’s phenotypic diversity index, the genetic multiple attitudes of wild population and cultured population were 0.214 and 0.201 respectively, and the genetic similarity between the two populations was 94.20 %, The genetic distance is 0.058.