论文部分内容阅读
目的:研究补肾方药对去卵巢骨质疏松雌鼠的治疗作用及其机制。方法:70只雌性SD大鼠分为5组:正常组、假手术组、模型组、雌激素组、补肾方药组。手术12周后开始给药,连续给药12周,放射免疫法(RIA)检测雌二醇(E2)、白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)含量,酶联免疫法(ELISA)检测转化生长因子-β1(TGF-β1)含量。结果:与模型组比较,补肾方药组血清E2显著升高(P<0.01),IL-1、TNF-α均显著减少(P<0.05,P<0.01),血清TGF-β1显著增加(P<0.01)。结论:补肾方药抗去卵巢雌鼠骨质疏松的作用机制可能与补肾方药可升高雌激素,下调IL-1和TNF-α表达,抑制骨吸收;同时上调TGF-β1表达,促进骨形成有关。
Objective: To study the therapeutic effect and its mechanism of Bushen Recipe on ovariectomized female rats. Methods: Seventy female SD rats were divided into 5 groups: normal group, sham operation group, model group, estrogen group and Bushen Fangyao group. After 12 weeks of operation, the rats were dosed for 12 weeks. The levels of E2, IL-1, TNF-α and The level of transforming growth factor-β1 (TGF-β1) was detected by ELISA. Results: Compared with the model group, serum E2 was significantly increased in Bushen Recipe group (P <0.01), IL-1 and TNF-α were significantly decreased (P <0.05, P <0.01) 0.01). Conclusion: The mechanism of Bushen Recipe in preventing osteoporosis in ovariectomized female rats may be related to that Bushen Recipe can increase estrogen, down-regulate the expression of IL-1 and TNF-α, restrain bone resorption, increase TGF-β1 expression and promote bone formation .