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本文将妊娠11天大鼠胚胎脑皮质组织移植到成年大鼠支配股二头肌的溃变神经外膜内,对移植的神经组织生存、分化、成熟的形态变化进行了超微结构研究。移植后7天,移植块由神经上皮细胞团所组成。细胞电子密度较高。细胞团周边由基膜复盖。14～21天,移植块细胞开始分散。核分化并趋向成熟,胞质内粗面内质网开始集聚,神经毯出现,胶质细胞开始成熟。30天,有成熟的神经细胞。核质电子密度低,核仁明显,粗面内质网多行排列,胶质细胞成熟。45天,神经元溃变,核质凝聚或溶解,粗面内质网消失,胶质细胞依然存在。60～120天,移植部仅余胶质细胞和一些雪旺氏细胞。实验结果说明胚胎脑皮质在溃变周围神经中至少在移植后一个月内已能分化、成熟和形成一个移位性的神经组织。同时还表明移植块的生存和移植部位神经组织结构有密切关系。本实验模型对于观察胚胎中枢神经系统移植块生长、分化、成熟等方面,具有一定的优点。
In this study, the embryonic cerebral cortex tissue of 11-day-old pregnant rats was transplanted into the degenerative nerve adventitia of the adductor biceps femoris in adult rats. The morphological changes of survival, differentiation and maturation of the transplanted nerve tissues were investigated by ultrastructure. Seven days after transplantation, the grafts consisted of neuroepithelial cell masses. Cell electron density is higher. Peripheral cells covered by the basement membrane. From 14 to 21 days, the engraftment cells began to disperse. Nuclear differentiation and maturation, endoplasmic reticulum began to gather cytoplasm, neuralgia blanket, glial cells began to mature. 30 days, there are mature nerve cells. Nuclear electron density is low, obvious nucleolus, rough endoplasmic reticulum arranged in rows, glial cells mature. 45 days, neuronal degeneration, condensation or dissolution of nuclear matter, the disappearance of rough endoplasmic reticulum, glial cells still exist. 60 to 120 days, only the Department of grafting glial cells and some Schwann cells. The experimental results show that the embryonic cerebral cortex can differentiate, mature and form a transplanted nerve tissue in the degenerated peripheral nerve at least within one month after transplantation. At the same time, it also shows that there is a close relationship between the survival of the graft and the nerve tissue of the transplantation site. The experimental model for the observation of embryonic central nervous system transplantation block growth, differentiation, maturation, etc., has some advantages.