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目的:探讨旋毛虫幼虫抗原免疫小鼠对日本血吸虫攻击感染的交叉保护作用。方法:用4种不同的旋毛虫幼虫抗原制剂经颈部皮下多点免疫小鼠,然后用日本血吸虫尾蚴30条或100条攻击感染,攻击后45d剖杀小鼠,收集成虫、肝组织和粪便中的虫卵,并计数。结果:4种不同制剂均可诱导不同程度的保护性免疫效应,以旋毛虫幼虫匀浆上清可溶性抗原(TsSA)效果较好,减虫率为21.3%,加用福氏佐剂时,其减虫率为29.3%,肝组织和粪便中的虫卵减少率分别达48%和58.5%,平均每鼠肝组织和粪便中的虫卵EPG减少率分别为41.7%和48.9%;当抗原剂量为10000条旋毛虫并加用福氏佐剂时,其减虫率达39.6%。结论:旋毛虫抗原免疫小鼠能诱导抗日本血吸虫攻击感染的免疫效应
Objective: To investigate the cross-protection of mice immunized with Trichinella spiralis antigen against challenge with Schistosoma japonicum. Methods: Mice were immunized subcutaneously with 4 different Trichinella spiralis larvae antigen preparations and then challenged with 30 or 100 challenged cercariae of Schistosoma japonicum. Mice were sacrificed 45 days after challenge to collect adult, liver and feces In the eggs, and count. Results: Four kinds of different preparations could induce protective immune effect to varying degrees. Trichomonas larvae homogenized supernatant soluble antigen (TsSA) was better, the worm reduction rate was 21.3%. When added with Freund’s adjuvant , The worm reduction rate was 29.3%, and the reduction rates of the eggs in liver tissue and feces were 48% and 58.5% respectively. The average EPG reduction rates in the liver tissue and feces of the mice were 41.7 % And 48.9% respectively. When the antigen dose was 10000 Trichinella spiralis and added with Freund’s adjuvant, the worm reduction rate was 39.6%. CONCLUSION: Immunization of mice with Trichinella antigens induces an immune response against infection with Schistosoma japonicum