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目的探讨用膜表面标志CD4+CD25+CD127low/-作为检测调节性T(Treg)细胞标记的可行性,并探讨其在系统性红斑狼疮(SLE)中的临床意义。方法用流式细胞术检测SLE组及健康对照组外周血CD4+CD25+CD127low/-Treg细胞及CD4+CD25+FoxP3+Treg细胞的比例,并分析两组CD4+CD25+CD127low/-Treg细胞与CD4+CD25+FoxP3+Treg细胞比例之间的相关性。结果SLE组外周血CD4+CD25+CD127low/-Treg细胞比例为(3.31±0.82)%,CD4+CD25+FoxP3+Treg细胞比例为(2.28±0.47)%,均显著低于健康对照组的(6.07±1.59)%和(5.01±1.09)%(P<0.01)。SLE组及健康对照组外周血CD4+CD25+CD127low/-Treg细胞比例与CD4+CD25+FoxP3+Treg细胞比例之间呈显著正相关(r=0.713、r=0.709,P<0.01)。结论膜表面标志CD4+CD25+CD127low/-可以用来鉴定Treg细胞;SLE患者外周血CD4+CD25+CD127low/-Treg细胞的显著减少可能与SLE的发病有关。
Objective To investigate the feasibility of using membrane surface marker CD4 + CD25 + CD127low / - as a marker of regulatory T (Treg) cells and to investigate its clinical significance in systemic lupus erythematosus (SLE). Methods Flow cytometry was used to detect the percentage of CD4 + CD25 + CD127low / -Treg cells and CD4 + CD25 + FoxP3 + Treg cells in peripheral blood of SLE group and healthy control group. The levels of CD4 + CD25 + CD127low / -Treg cells and Correlation of CD4 + CD25 + FoxP3 + Treg Cell Ratio. Results The percentage of CD4 + CD25 + CD127low / -Treg cells in peripheral blood was (3.31 ± 0.82)% in SLE group and (2.28 ± 0.47)% in CD4 + CD25 + FoxP3 + Treg cells in SLE group, ± 1.59)% and (5.01 ± 1.09)%, respectively (P <0.01). There was a significant positive correlation between CD4 + CD25 + CD127low / -Treg cells and CD4 + CD25 + FoxP3 + Treg cells in peripheral blood of SLE group and healthy control group (r = 0.713, r = 0.709, P <0.01). Conclusion The membrane surface marker CD4 + CD25 + CD127low / - can be used to identify Treg cells. The significant reduction of CD4 + CD25 + CD127low / -Treg cells in peripheral blood of patients with SLE may be related to the pathogenesis of SLE.