尿铅测定是铅中毒诊断、铅作业工人预防体检常用指标。测定方法有双硫腙分光光度法,原子吸收分光光度法等。作者等曾介绍四-(对-三甲铵苯基)卟啉分光光度法测定空气铅。在此基础上又摸索了应用甲基异丁酮萃取、四-(对-三甲铵苯基)卟啉分光光度法测定尿铅,并经过实际样品测试,还与双硫腙法进行了比较。 方法 1、试剂 0.02％四-(对-三甲铵苯基卟啉溶液,简称TAPP)。铅标准贮备液1mg/ml。10％氰化钾。2％硝酸。0.1M四硼酸钠溶液。饱和碘化钾溶液。2.5％辛基苯氧基聚乙氧基乙醇溶液,简称(Tx-100)。0.25M氢氧化钠溶液。甲基异丁酮,简称MIBK。以上试剂为分析 Determination of lead in urine lead poisoning diagnosis, lead workers to prevent physical examination commonly used indicators. Determination of dithizone spectrophotometry, atomic absorption spectrophotometry. The authors have previously described the determination of air lead by tetrakis (p-trimethylammoniunophenyl) porphyrin spectrophotometry. On this basis, we explored the use of methyl isobutyl ketone extraction, tetra - (p - trimethylammonium phenyl) porphyrin spectrophotometric determination of lead in urine, and after the actual sample test, but also with the dithizone method were compared. Method 1, reagent 0.02% tetra- (p-trimethylammoniophenyl porphyrin solution, referred to as TAPP). Lead standard stock solution 1mg / ml. 10% potassium cyanide. 2% nitric acid. 0.1M sodium tetraborate solution. Saturated potassium iodide solution. 2.5% octyl phenoxy polyethoxy ethanol solution, referred to as (Tx-100). 0.25M sodium hydroxide solution. Methyl isobutyl ketone, referred to as MIBK. The above reagents for analysis