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The silicate colloids with an average diameter 100 nm, were prepared by the hydrolysis of tetraethoxysilane (TES), NH4OH (30%) and then modified by (3-mercaptopropyl) trimethoxysilane ( APS). The colloids can adsorb heavy metals such as Pb and Cr in effluent and after adsorption the colloids can be separated by coagulation of aluminum sulfate. The removal of heavy metals is up to 99%.