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目的研究长链非编码RNA(lncRNA)母系印记基因3(MEG3)对人类流产绒毛发育的影响,并探讨其分子机制。方法收集自然流产及人工流产各15例患者的绒毛组织,使用免疫组化方法检测其凋亡因子Bax及凋亡抑制因子Bcl-2的表达,使用qPCR法检测MEG3的表达。在人滋养细胞系HTR-8/SVneo细胞中过表达MEG3后,利用qPCR法检测MEG3的表达,利用细胞侵袭实验比较过表达组与对照组细胞的侵袭能力。结果人工流产绒毛组织中Bax的表达低于自然流产绒毛,而Bcl-2的表达高于自然流产绒毛(P<0.01)。人工流产绒毛组织中的MEG3的表达低于自然流产(P<0.01)。人滋养层细胞系HTR-8/SVneo细胞中过表达MEG3后,HTR-8/SVneo细胞侵袭能力降低(P<0.01)。结论 lncRNA MEG3可调控滋养层细胞的凋亡及侵袭能力,影响人类绒毛的发育。
Objective To investigate the effect of long-chain non-coding lncRNA maternal imprinting gene 3 (MEG3) on the development of human hair loss and to explore its molecular mechanism. Methods Villus tissue was collected from 15 patients with spontaneous abortion and induced abortion. The expression of Bax and apoptosis inhibitor Bcl-2 were detected by immunohistochemistry. The expression of MEG3 was detected by qPCR. After overexpression of MEG3 in human trophoblast cell line HTR-8 / SVneo, the expression of MEG3 was detected by qPCR and the invasion ability of cells in overexpression and control groups was compared by cell invasion assay. Results The expression of Bax in artificial abortion villus was lower than that of spontaneous abortion, while the expression of Bcl-2 was higher than that of spontaneous abortion (P <0.01). The expression of MEG3 in artificial abortion chorionic tissue was lower than that of spontaneous abortion (P <0.01). The overexpression of MEG3 in human trophoblast cell line HTR-8 / SVneo decreased the invasiveness of HTR-8 / SVneo cells (P <0.01). Conclusion lncRNA MEG3 regulates the apoptosis and invasion of trophoblast cells and affects the development of human villi.